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Guinea pigs are popular pets known for their adorable appearance and gentle nature. For many pet owners, accurately determining the sex of their guinea pig is an important task. Whether you are considering breeding or simply want to ensure proper care and socialization, understanding the gender of your guinea pig is crucial. However, identifying the sex of these small rodents can sometimes be a challenging task. In this guide, we will explore various techniques and characteristics that can help you determine the sex of a guinea pig with confidence. From examining anatomy to observing behavioral cues, we will provide you with the necessary knowledge and expertise to accurately determine the sex of your beloved furry friend.
This article was co-written by Pippa Elliott, MRCVS. Dr. Elliott is a veterinarian with over thirty years of experience. She graduated from the University of Glasgow in 1987 and worked as a veterinary surgeon for 7 years. Then, Dr. Elliott worked as a veterinarian in a clinic for over a decade.
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Many guinea pig owners have difficulty determining their sex, especially with newborn guinea pigs. [1] XResearch Source Sex determination of your guinea pig can help you prevent unwanted pregnancy when other mice are brought into the colony and determine if the pups need to be separated from the mother. [2] XResearch Source Although many veterinary clinics offer guinea pig sexing services, you can also do this yourself.
Steps
Check guinea pig

- Determining the sex of very young guinea pigs is extremely difficult. You can wait until the rat is 2-3 weeks old, at which point sex determination will be much easier [3] XResearch Source . However, since males need to be separated from their mother and herd at 3 weeks of age, you must have their sexes sorted before this point, otherwise the males may mate with the mother and other animals. his sisters.
- Don’t worry about coming into contact with baby guinea pigs. Their mothers will not abandon their children and the young will also be bolder when they are exposed to humans early. [4] XResearch Sources

- You don’t need to wear gloves. Guinea pigs rarely transmit disease from humans, and gloves are not very helpful in preventing disease from spreading from one individual to another in the rat colony.


- Baby guinea pigs don’t like to stay on their backs for too long, so try to get them checked out quickly and efficiently. You can ask someone else to hold the mouse down for faster checking.
- Give your guinea pig food while you’re checking. Rats will cooperate and be less afraid.
Determine the sex of the mouse

- Once you’ve identified the guinea pig’s anus and external genitalia, you’ll want to consider the distance between them. The distance from the vulva to the anus of female guinea pigs is quite short, usually their vulva will be located just above the anus. The distance from the penis to the anus of the male guinea pig will be larger, they are usually about 5-7.5 cm apart.

- The male guinea pig’s genital opening is shaped like a small round dot with the penis protruding beyond the surrounding skin.
- There is a bulge in the area between the genitals and anus of the male guinea pig. This bulge occurs because the rat’s testicles are located just below the skin, along the anus and penis. You should also check to see if there is a raised circle around the anus of the mouse, which is the location of the base of the testicle.

- You can also use your fingers to gently press and rub just above the genitals. If you feel a ‘ripple’, it’s the inside of the penis and you can be sure it’s a male guinea pig.
- While examining your rat’s penis, look for testicles on either side of the penis to make sure the guinea pig is a male. The testicles of guinea pigs are quite large for their body size.

- You should take your guinea pig to the veterinarian if, during sex testing, the rat appears upset or uncooperative. If your guinea pig is stressed or struggling too much during a home sex test, it will be difficult for you to get an accurate result.
This article was co-written by Pippa Elliott, MRCVS. Dr. Elliott is a veterinarian with over thirty years of experience. She graduated from the University of Glasgow in 1987 and worked as a veterinary surgeon for 7 years. Then, Dr. Elliott worked as a veterinarian in a clinic for over a decade.
This article has been viewed 16,300 times.
Many guinea pig owners have difficulty determining their sex, especially with newborn guinea pigs. [1] XResearch Source Sex determination of your guinea pig can help you prevent unwanted pregnancy when other mice are brought into the colony and determine if the pups need to be separated from the mother. [2] XResearch Source Although many veterinary clinics offer guinea pig sexing services, you can also do this yourself.
In conclusion, determining the sex of a guinea pig can be a straightforward process if one knows what to look for. By observing external genitalia, such as the distance between the anus and genital opening, and the presence or absence of a Y-shaped or straight line, one can determine whether a guinea pig is male or female. However, it is essential to be cautious as misidentifying the sex can lead to unintended consequences, such as accidental breeding or improper care. It is always recommended to consult a veterinarian or a knowledgeable guinea pig breeder for accurate sex determination, especially in cases of young or unfamiliar guinea pigs. Additionally, while knowing the sex of a guinea pig is valuable for various reasons, such as separating males and females to prevent breeding or specific medical considerations, it is important to remember that the sex of the guinea pig does not define its personality, care needs, or the joy it can bring as a beloved pet.
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