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Molar absorbance is a crucial concept in analytical chemistry and spectroscopy. It refers to the absorption of a substance at a particular wavelength, and is directly proportional to the concentration of the substance. By calculating the molar absorbance, scientists and researchers can determine the concentration of a specific compound in a solution using spectrophotometry. This introduction will present an overview of the steps involved in calculating molar absorbance, highlighting the significance of this calculation in various scientific fields.
This article was co-written by Bess Ruff, MA. Bess Ruff is a graduate student in geography at Florida. She received her Master’s degree in Environmental Science and Management from the Bren School of Environmental Science & Management, UC Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the coastal area. Caribbean and support research as a contributor to the Sustainable Fisheries Group.
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Absorbance mp, also known as molecular extinction factor, is a measure of how well a chemical absorbs optically at a given wavelength of light. It allows you to compare compounds with each other without taking into account differences in concentration or solution thickness during measurement. [1] XResearch Source The absorbance mp is commonly used in chemistry, and should not be confused with the more commonly used extinction factor in physics. The standard unit of absorbance mp is liters per mp centimeter (L mp -1 cm -1 ). [2] XResearch Source
Steps
Calculate the absorption coefficient mp using the formula
- Absorbance can be calculated as the ratio between the incident light intensity and the emergent light intensity. We have the formula A = log 10 (I o /I) . [4] XResearch Sources
- The intensity of the light is measured by a spectrophotometer.
- The absorbance of the solution will change with the wavelength of light passing through the solution. Some wavelengths will be absorbed more than others, depending on the composition of the solution. Remember to write down the wavelength you are using for the calculation. [5] XResearch Sources
- The absorbance between measurements may vary due to the concentration of the solution and the shape of the vessel used to measure the intensity. The absorbance mp will compensate for these errors. [6] XResearch Source
- Prepare a solution of known concentration c for analysis. The unit of concentration is mp/liter. [7] XResearch Sources
- To find l , we measure the thickness of the cuvette. The unit of transmitted thickness is centimeters.
- Using a spectrophotometer measure the absorbance A at a given wavelength. The unit of wavelength is the meter, but most wavelengths are so small that nanometers (nm) are used. [8] XResearch Source Absorbance is a dimensionless quantity.
- Example: Using a cuvette thickness of 1 cm, measure the absorbance of a solution with a concentration of 0.05 mp/L. The absorbance at 280 nm is 1.5. Calculate the absorbance mp of the solution?
- 280 = A/lc = 1.5/(1 x 0.05) = 30 L mp -1 cm -1
Calculate the absorbance mp using the standard curve method
- Draw a straight line through each point. If the measured values are correct, the points will form a straight line showing that the absorbance and concentration are directly proportional to Beer’s law. [10] XResearch Source
- The slope equation of the line is (Y 2 – Y 1 )/(X 2 – X 1 ). The higher point on the line is denoted by a small 2 below, and the lower point is denoted by 1.
- Example: The absorbance of a solution with concentrations of 0.2 mp and 0.3 mp is 0.27 and 0.41, respectively. The absorbance is the Y value while the concentration is the X value. Use the straight-line equation (Y 2 – Y 1 )/(X 2 – X 1 ) = (0.41-0.27)/(0) ,3-0.2) = 0.14/0.1 = 1.4 is the slope of the line.
- Continuing with the above example: If the slope of the line is 1.4 and the transmission thickness is 0.5 cm, the absorbance mp is 1.4/0.5 = 2.8 L mp -1 cm -1 .
This article was co-written by Bess Ruff, MA. Bess Ruff is a graduate student in geography at Florida. She received her Master’s degree in Environmental Science and Management from the Bren School of Environmental Science & Management, UC Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the coastal area. Caribbean and support research as a contributor to the Sustainable Fisheries Group.
This article has been viewed 46,248 times.
Absorbance mp, also known as molecular extinction factor, is a measure of how well a chemical absorbs optically at a given wavelength of light. It allows you to compare compounds with each other without taking into account differences in concentration or solution thickness during measurement. [1] XResearch Source The absorbance mp is commonly used in chemistry, and should not be confused with the more commonly used extinction factor in physics. The standard unit of absorbance mp is liters per mp centimeter (L mp -1 cm -1 ). [2] XResearch Source
In conclusion, calculating molar absorbance is a crucial step in determining the concentration of a substance in a solution. By utilizing Beer-Lambert’s Law, which states that absorbance is directly proportional to concentration, one can accurately determine the amount of a substance present in a solution. The molar absorbance is calculated by dividing the absorbance by the path length and molar concentration. This calculation allows scientists to quantify the amount of a substance and analyze its behavior in different solutions. Additionally, molar absorbance calculations are widely used in various scientific fields such as chemistry, biology, and environmental sciences. Understanding how to calculate molar absorbance is essential for accurate analysis and can greatly contribute to the advancement of scientific research.
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